RNAi介导的HOXD3敲低抑制人RKO细胞的生长

摘要:2016年3月,安徽医科大学**附属医院肿瘤科;安庆医学院临床医学系;安徽医科大学附属安庆市医院病理科(Department of Oncology, The First Affiliated Hospital of Anhui Medical Univers

2016年3月,安徽医科大学**附属医院肿瘤科;安庆医学院临床医学系;安徽医科大学附属安庆市医院病理科(Department of Oncology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022; Department of Clinical Medicine, Anqing Medical College, Anqing, Anhui 246052; Department of Pathology, The Affiliated Anqing Municipal Hospital of Anhui Medical University, Anqing, Anhui 246003, P.R. China) FANGJUN CHEN老师研究团队在《ONCOLOGY REPORTS》上发表论文:

“RNAi-mediated HOXD3 knockdown inhibits growth in human RKO cells”

“RNAi介导的HOXD3敲低抑制人RKO细胞的生长”

Abstract:

Numerous studies have shown that the multifunctional Homeobox-containing (HOX) D3 gene is involved in various physiological and pathological processes. To elucidate the role and mechanism of HOXD3 in colorectal cancer (CRC), we measured its expression in five CRC cell lines. After determining that HOXD3 was highly expressed in the human RKO cancer cell line, we used lentiviral-mediated small interfering RNAs (siRNAs) to knock down HOXD3 expression and assessed proliferation, cell cycle distribution, apoptosis and colony formation using cell proliferation, flow cytometric, and colony formation assays. The expression of HOXD3 was strongly suppressed in the RKO cells infected with the lentiviruse expressing an HOXD3 short hairpin RNA (shRNA). The downregulation of HOXD3 expression in RKO cells significantly decreased proliferation and colony formation, and increased apoptosis in vitro, compared to the cells infected with the mock control (p

摘要:

大量研究表明,多功能同源盒(HOX) D3基因参与多种生理和病理过程。为了阐明HOXD3在结直肠癌(CRC)中的作用和机制,我们检测了其在5种结直肠癌细胞系中的表达。在确定HOXD3在人类RKO癌细胞系中高表达后,我们使用慢病毒介导的小干扰rna (sirna)来敲低HOXD3的表达,并使用细胞增殖、流式细胞术和集落形成试验来评估增殖、细胞周期分布、凋亡和集落形成。表达HOXD3短发夹RNA (shRNA)的慢病毒感染RKO细胞后,HOXD3的表达被强烈抑制。与模拟对照组相比,下调HOXD3表达可显著降低RKO细胞的体外增殖和集落形成,增加细胞凋亡(p

该论文中,DLD-1、HCT-116、SW620、HT-29和RKO人结肠癌细胞系的体外培养是使用Ausbian特级胎牛血清完成的。欲了解或购买Ausbian特级胎牛血清可以联系北京缔一生物400-166-8600.

来源:周瑞简说科学

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