内源性瘦素促进ebss诱导的PFCs自噬

摘要：2019年8月，云南省异种移植工程研究中心；云南省异种移植工程研究中心；云南省生物资源保护与利用国家重点实验室；云南农业大学；云南农业大学；动物科学与技术学院；云南农业大学动物医学院(Key Laboratory of Animal Gene Editing

2019年8月，云南省异种移植工程研究中心；云南省异种移植工程研究中心；云南省生物资源保护与利用国家重点实验室；云南农业大学；云南农业大学；动物科学与技术学院；云南农业大学动物医学院(Key Laboratory of Animal Gene Editing and Animal Cloning in Yunnan Province, Kunming, People’s Republic of China；Xenotransplantation Engineering Research Center in Yunnan Province, Kunming, People’s Republic of China；State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, People’s Republic of China；College of Animal Science and Technology,Yunnan Agricultural University, Kunming, People’s Republic of China；College of Veterinary Medicine, Yunnan Agricultural University, Kunming，People’s Republic of China) Hong-Ye Zhao老师研究团队在《ANIMAL CELLS AND SYSTEMS》上发表论文：

“Endogenous leptin promotes autophagy in EBSS-induced PFCs”

“内源性瘦素促进ebss诱导的PFCs自噬”

Abstract：

Leptin is an important adipokine and plays a vital role in animals. However, the role of leptin in the autophagic response of pig fibroblast cells (PFCs) has not been fully elucidated. In this study, we investigated the relationship between leptin and autophagy as well as underlying molecular basis. We found that PFCs treated with EBSS could secrete leptin, and the leptin concentration in the supernatant of leptin transgenic PFCs was higher than that of WT PFCs. We found an increase in LC3-II protein level and a decrease in p62 protein level in treated leptin transgenic PFCs compared with treated WT PFCs. Meanwhile, we observed an increase of autophagosomes by transmission electron microscopy and an enhancement of the accumulation of LC3 puncta in the cytoplasm of treated leptin transgenic PFCs, and these effects were further augmented by Baf A1 treatment. Furthermore, we detected the expression levels of 7 autophagy signaling pathway genes and 17 autophagy-related (ATG) genes by q-PCR. We found that between the two types of EBSS-treated cells 3 genes expression pattern were significantly different among the 7 autophagy signaling pathway genes and 8 genes expression pattern were significantly differernt among the ATG genes. These results indicated that leptin may promote autophagy and involving the downregulation of FOXO1 and LMNA genes via an unknown pathway which causes the upregulation of the 4 genes and the downregulation of 4 genes.

摘要：

瘦素是一种重要的脂肪因子，在动物体内起着至关重要的作用。然而，瘦素在猪成纤维细胞(PFCs)自噬反应中的作用尚未完全阐明。在这项研究中，研究人员探讨了瘦素与自噬的关系及其潜在的分子基础。研究人员发现经EBSS处理的pfc能够分泌瘦素，并且在瘦素转基因pfc的上清液中瘦素浓度高于WT pfc。研究人员发现，与处理过的WT pfc相比，处理过的瘦素转基因pfc中LC3-II蛋白水平升高，p62蛋白水平降低。同时，研究人员通过透射电镜观察到，处理过的瘦素转基因pfc细胞质中自噬体的增加和LC3点的积累增强，这些作用在Baf A1处理下进一步增强。此外，研究人员通过q-PCR检测了7个自噬信号通路基因和17个自噬相关(autophagy-related, ATG)基因的表达水平。研究人员发现在ebss处理的两种细胞中，7个自噬信号通路基因中有3个基因的表达模式存在显著差异，ATG基因中有8个基因的表达模式存在显著差异。这些结果表明，瘦素可能通过未知的途径促进自噬，并参与FOXO1和LMNA基因的下调，导致4个基因的上调和下调。

该论文中，WT和瘦素转基因PFCs细胞的体外培养是使用Ausbian特级胎牛血清完成的。欲了解或购买Ausbian特级胎牛血清可以联系北京缔一生物400-166-8600.