摘要：2018年3月，内蒙古医科大学附属医院(Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia, People’s Republic of China) Yun

2018年3月，内蒙古医科大学附属医院(Inner Mongolia Medical University Affiliated Hospital, Hohhot, Inner Mongolia, People’s Republic of China) Yunfei Bai老师研究团队在《Biosci Rep》上发表论文：

“Golgi integral membrane protein 4 manipulates cellular proliferation, apoptosis, and cell cycle in human head and neck cancer”

“高尔基整体膜蛋白4调控头颈癌细胞增殖、凋亡和细胞周期”

Abstract：

The incidence of global head and neck cancer has increased markedly in the last 10 years, and its prognosis is poor, which seriously endangers people's life and health. At present, there are few studies on its pathogenesis. Golgi integral membrane protein 4 (GOLIM4) is a major member of the Golgi apparatus transporter complex, and its role in tumor is unclear. The present study found that GOLIM4 was the key target protein downstream of stromal interaction molecule 1 (STIM1), which can inhibit the proliferation of head and neck cancer cells FaDu (human pharyngeal squamous carcinoma cell) and Tca-8113 (human tongue squamous carcinoma cell) with knockdown of GOLIM4 by lentivirus. And the decreased expression of GOLIM4 induced cellular apoptosis. Further experiments revealed that FaDu cell cycle progression was changed after GOLIM4 silence, G1
phase arrest and the number of G2/M cells decreased significantly. It was also found that the cells in S-phase decreased markedly after GOLIM4 was knocked down compared with the control group by 5-bromo-2'-deoxyuridine (BrdU) incorporation experiment. In conclusion, we found that GOLIM4, as the target gene downstream of STIM1, inhibited the proliferation of head and neck cancer, promoted apoptosis, and regulated cell cycle progression, and GOLIM4 is a novel oncogene in head and neck cancer and might help in developing promising targetted therapies for head and neck cancer patients.

摘要：

近10年来，全球头颈癌发病率显著上升，预后较差，严重危害人们的生命健康。目前对其发病机制的研究较少。高尔基整体膜蛋白4 (GOLIM4)是高尔基体转运体复合体的主要成员，其在肿瘤中的作用尚不清楚。本研究发现GOLIM4是基质相互作用分子1 (STIM1)下游的关键靶蛋白，可以通过慢病毒敲低GOLIM4抑制头颈部癌细胞FaDu(人咽鳞癌细胞)和Tca-8113(人舌鳞癌细胞)的增殖。而GOLIM4的表达降低可诱导细胞凋亡。进一步实验发现，GOLIM4沉默、G1期阻滞后，FaDu细胞周期进程发生改变，G2/M细胞数量明显减少。5-溴-2′-脱氧尿苷(BrdU)掺入实验也发现，与对照组相比，GOLIM4敲除后s期细胞明显减少。综上所述，科研人员发现GOLIM4作为STIM1下游的靶基因，可以抑制头颈癌的增殖，促进细胞凋亡，调节细胞周期进程，GOLIM4是头颈癌的一个新的致癌基因，可能有助于开发头颈癌患者的靶向治疗方法。

该论文中，FaDu(人咽鳞癌细胞)和Tca-8113(人舌鳞癌细胞)的体外培养是使用Ausbian特级胎牛血清完成的。欲了解或购买Ausbian特级胎牛血清可以联系北京缔一生物400-166-8600.

来源：科学话你知