摘要：2020年12月，山东第一医科大学附属山东省医院血液科；山东省精神卫生中心信息中心（Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medi

2020年12月，山东第一医科大学附属山东省医院血液科；山东省精神卫生中心信息中心（Department of Hematology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, People’s Republic of China;Information Center, Shandong Mental Health Center, Jinan, Shandong 250014, People’s Republic of China）Xin Wang老师研究团队在《Cancer Management and Research》上发表论文：

“Expression and Importance of TMED2 in Multiple Myeloma Cells”

“TMED2在多发性骨髓瘤细胞中的表达及意义”

Abstract

Objective: TMED2 is a member of the transmembrane emp24 domain (Tmed)/p24 protein family, which is significantly upregulated in breast cancer, ovarian cancer and other tumour tissues. The purpose of this study was to investigate the expression of TMED2 in MM cell lines and its effect on the biological behaviour of MM cell lines.

Methods: Real-time quantitative PCR (RT-qPCR) was used to detect the expression of TMED2 in MM cell lines, including MM.1S and RPMI 8226 cells, and lentivirus vector-mediated TMED2 gene silencing was used to further study the effect of the downregulation of TMED2 expression on cell viability, the cell cycle, and apoptosis.

Results: Based on the RT-qPCR results, the expression of the TMED2 mRNA was increased in the MM cell lines MM.1S and RPMI 8226 compared with endogenous control GAPDH. The expression of the TMED2 mRNA was substantially reduced after transfection of the shRNA targeting TMED2 (shTMED2) in both MM cell lines. The CCK-8 assay showed significant decreases in the viability of MM.1S and RPMI 8226 cells, suggesting that the TMED2 gene plays an important role in the proliferation of these two cell lines. The cell cycle of MM.1S and RPMI 8226 cells was substantially altered by shTMED2, as evidenced by the increased number of cells in G1 phase and decreased number of cells in S and G2/M phases. The FACS analysis revealed a significant increase in the apoptosis of MM.1S and RPMI 8226 cells due to the increased activity of Caspase 3/7, suggesting that the TMED2 gene is significantly related to the apoptosis of these two cell lines.

Conclusion: Based on these results, TMED2 may play an important role in the pathogenesis of MM. This novel study may contribute to further investigations of useful biomarkers and potential therapeutic targets in patients with MM.

摘要

目的:TMED2是跨膜emp24结构域(Tmed)/p24蛋白家族成员，在乳腺癌、卵巢癌等肿瘤组织中显著上调。本研究旨在探讨TMED2在MM细胞株中的表达及其对MM细胞株生物学行为的影响。

方法:采用实时荧光定量PCR (RT-qPCR)检测MM细胞系MM. 1s和RPMI 8226细胞中TMED2的表达，采用慢病毒载体介导的TMED2基因沉默，进一步研究下调TMED2表达对细胞活力、细胞周期和凋亡的影响。

结果:RT-qPCR结果显示，与内源性对照GAPDH相比，MM细胞系MM. 1s和RPMI 8226中TMED2 mRNA的表达增加。在两种MM细胞系中转染靶向TMED2的shRNA (shTMED2)后，TMED2 mRNA的表达均显著降低。CCK-8检测显示MM.1S和RPMI 8226细胞的活力显著降低，提示TMED2基因在这两种细胞系的增殖中起重要作用。shTMED2明显改变了MM.1S和RPMI 8226细胞的细胞周期，表现为G1期细胞数量增加，S期和G2/M期细胞数量减少。FACS分析显示，由于Caspase 3/7活性的增加，MM.1S和RPMI 8226细胞的凋亡明显增加，提示TMED2基因与这两种细胞系的凋亡有显著关系。

结论:基于这些结果，TMED2可能在MM的发病机制中发挥重要作用，本研究可能有助于进一步探索MM患者有用的生物标志物和潜在的治疗靶点。

该论文中，人骨髓瘤细胞系RPMI 8226和MM.1S细胞的体外培养是使用Ausbian特级胎牛血清完成的。

来源：科学巨匠